【UKUSETYENZISWA OKUHLOSIWEYO】

I-RSV Rapid Test Device yi-in vitro diagnostic test for the qualitative discovery of Respiratory Syncytial Virus antigens kwi-Nasal Swab kunye neesampuli ze-aspirate zempumlo, usebenzisa indlela ye-immunochromatographic ekhawulezayo.Ukuchonga kusekelwe kwii-antibodies ze-monoclonal ezithile kwiprotheni ye-F ye-Respiratory Syncytial Virus. Iya kunika ulwazi koogqirha bezonyango ukuba bamisele amayeza achanekileyo. Iziphumo ezibi kufuneka ziqinisekiswe ngezinye iindlela, ezifana nenkcubeko yeeseli.

【USHWANKATHELO】

I-RSV yintsholongwane enye engalunganga-enomtya weRNA yosapho lweParamyxoviridae1. Ingunobangela wosulelo olusulelayo kakhulu, olubukhali, lwentsholongwane egazini lomgudu wokuphefumla. Ehlabathini lonke, iRSV inegalelo ekuswelekeni kweentsana eziya kutsho kwi-1,000,000 kunye nabantwana abangaphantsi kweminyaka emihlanu ngonyaka2. Ikwangunobangela omkhulu wentsholongwane wentsholongwane wesifo se-nosocomial kubantwana abasele belele esibhedlele ngenxa yezinye izizathu3. Phakathi kwabantwana abalaliswe esibhedlele ngosulelo lwe-RSV, izinga lokufa liqikelelwa ukuba liphantsi njenge-0.3% ukuya kwi-1.0% 4 kunye noluhlu lwe-2.5% ukuya kwi-4.0% kubantwana abane-cardiac ephantsi okanye isifo se-pulmonary5. Usulelo lwe-RSV lunika kuphela ukhuselo lomzimba olulinganiselweyo. Ngoko ke, abantu bangosuleleka ngokuphindaphindiweyo kwaye babe nesifo esimandundu kubo bonke ubomi babo6.

I-RSV ine-serotype enye kuphela, kwaye ihlukaniswe ngamacandelo amabini e-antigenic, A kunye ne-B7.Amaqela omabini ajikeleza ngaxeshanye kuluntu. Udidi olungaphantsi lwe-B luphawulwa njengohlobo lwentsholongwane olungenazimpawu zesifo esifunyanwa sisininzi sabemi. Izigulo zeklinikhi eziqatha kakhulu zibandakanya iintlobo ezingaphantsi kohlobo A, oluthanda ukongamela kuqhambuko oluninzi. I-RSV inokukrokrelwa ngokusekelwe kwixesha lonyaka losulelo; ukuxhaphaka kudla ngokungqamana nexesha lomkhuhlane wasebusika.

I-RSV inokuvavanywa kwilebhu kusetyenziswa iimfihlo zokuphefumla.Uvakalelo lokwahlulwa kwentsholongwane kwiimfihlo zokuphefumla kwinkcubeko yeeseli kuyahluka phakathi kweelabhoratri.Uvavanyo olunovakalelo oluphezulu lwe-RT-PCR kufuneka luqwalaselwe xa kuhlolwa abantu abadala, kuba banokuba nomthamo wentsholongwane egazini osezantsi kwimizekelo yabo yokuphefumla. Nangona iluncedo kuphando, iimvavanyo zeserologic azisetyenziswa rhoqo ukuxilongwa. I-Seroconversion ayenzeki ubuncinane kwiiveki ze-2 kwaye inokufuna iiveki ze-4 ~ 6. Uninzi lweelebhu zeklinikhi ngoku zisebenzisa iimvavanyo zokubona i-antigen. Iimvavanyo zokufumanisa i-Antigen kunye nenkcubeko zithembeke ngokubanzi kubantwana abancinci kodwa zincinci kubantwana abadala nakubantu abadala.

I-RSV ye-Antigen Rapid Test Device yindlela elula yokuhlola i-lateral flow immunoassay yokukhangela ngokuthe ngqo ii-antigens ze-RSV. Iya kubonelela ngoxilongo olucingelwayo losulelo lwe-RSV.

【IZIKHOKELO ZOKUSETYENZISWA】

Vumela isixhobo sovavanyo, isampulu yovavanyo kunye nesithinteli ukuba silingane nobushushu begumbi (15-30°C) phambi kovavanyo. Nceda ujonge ikhadi lenkqubo kule khithi.

1) Susa isixhobo sovavanyo kwisingxobo esitywiniweyo ngaphambi nje kovavanyo kwaye ulale phantsi kwibhentshi yomsebenzi.

2) Faka umbhobho ngesihluzo kwityhubhu yokutsalwa kwesampulu ngokuqinileyo.

3) Buyisela umva ityhubhu yokutsalwa kwesampulu, kwaye wongeze i-3 yehla (malunga ne-100μl) yesampula yovavanyo ngokucinezela ityhubhu yesisombululo ekhutshiweyo kwisampulu yefestile.

4) Funda iziphumo ngemizuzu eyi-15.