【TAKAITACCEN】

Myoglobin (MYO), Creatine Kinase MB (CK - MB) da Troponin na zuciya (cTnI) sune sunadaran da aka saki a cikin jini bayan rauni na zuciya. Myoglobin heme - furotin da aka saba samu a cikin kwarangwal da tsokar zuciya tare da nauyin kwayoyin halitta na 17.8 kDa. Lokacin da ƙwayoyin tsoka suka lalace, Myoglobin yana fitowa cikin jini cikin sauri saboda ƙananan girmansa. Matsayin Myoglobin yana ƙaruwa sama da tushe a cikin awanni 2 CK-MB wani enzyme ne kuma yana samuwa a cikin tsokar zuciya, tare da nauyin kwayoyin halitta na 87.0 kDa. Creatine Kinase kwayar halitta ce ta dimeric da aka samu daga sassa biyu da aka tsara a matsayin "M" da "B", wanda ya haɗu ya zama isoenzymes daban-daban guda uku, CK-MM, CK-BB da CK-MB. CK-MB shine isoenzyme na Creatine Kinase mafi yawan shiga cikin metabolism na ƙwayar tsoka na zuciya. Ana iya gano sakin CK-MB cikin jini bayan MI a cikin awanni 3-8 bayan bayyanar cututtuka. Yana girma a cikin sa'o'i 9 zuwa 30, kuma yana komawa zuwa matakan asali a cikin sa'o'i 48 zuwa 72. Cardiac Troponin I shine furotin da ake samu a tsokar zuciya, tare da nauyin kwayoyin halitta na 22.5 kDa. Troponin I wani bangare ne na hadaddun rukunoni guda uku wanda ya kunshi Troponin T da Troponin C. Tare da tropomyosin, wannan hadadden tsarin ya zama babban bangaren da ke daidaita ayyukan ATPase na Calcium na actomyosin a cikin skeletal da tsoka na zuciya. Bayan raunin zuciya ya faru, an saki Troponin I cikin jini 4-6 hours bayan fara jin zafi. Tsarin sakin Troponin I yana kama da CK-MB, amma yayin da matakan CK-MB suka dawo daidai bayan sa'o'i 72, Troponin I ya kasance mai girma har tsawon kwanaki 6-10, don haka yana samar da taga mai tsawo don gano ciwon zuciya.

Myoglobin/CK-MB/Troponin I Combo Rapid Test Cassette (Dukkan Jini/Serum/Plasma) gwaji ne mai sauƙi wanda ke amfani da haɗe-haɗe na barbashi na antibody da kama reagents don gano Myoglobin, CK-MB da cardiac Troponin I (cTnI) a cikin jini gabaɗaya, ko jini. Matsakaicin matakin ganowa shine 50ng/mL Myoglobin, 5ng/mL CK-MB da 0.5ng/mL Troponin I.

 【HANYOYIN AMFANI】

Bada kaset ɗin gwaji, samfuri, buffer da/ko sarrafawa don isa zafin ɗaki (15-30°C) kafin gwaji.

1. Kawo jakar zuwa zafin jiki kafin buɗe shi. Cire kaset ɗin gwajin daga jakar da aka rufe kuma yi amfani da shi da wuri-wuri.

2. Sanya kaset akan tsaftataccen wuri mai daidaitacce.

Don samfurin Serum ko Plasma:

● Rike digo a tsaye sannan a canja wurin digo 2 na jini ko plasma (kimanin 50µL) zuwa samfurin da kyau, sannan ƙara digo 1 na buffer (kimanin 40µL), sannan fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.

Ga Venipuncture Gabaɗayan Samfuran Jini:

· Rike digo a tsaye sannan a canja wurin digo 2 na duka jini (kimanin 50µL) zuwa samfurin da kyau, sannan a kara digo 1 na buffer (kimanin 40µL), sannan a fara mai lokaci. Dubi hoton da ke ƙasa.

Don Samfuran Dukan Jini na Yatsa:

Don amfani da bututun capillary: Cika bututun capillary kuma canja wurin kusan 50µL na ɗan yatsa gabaɗayan samfurin jini zuwa rijiyar kaset ɗin gwaji, sannan ƙara digo 1 na buffer (kimanin 40µL) kuma fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.

Don amfani da digon rataye: Bada digo biyu na rataye na ɗan yatsa gabaɗayan samfurin jini (kimanin 50 µL) su faɗi cikin samfurin kaset ɗin gwaji, sannan ƙara digo 1 na buffer (kimanin 40µL) kuma fara mai ƙidayar lokaci. Dubi hoton da ke ƙasa.

3. Jira layin (s) masu launi ya bayyana. Karanta sakamakon a minti 10. Kar a fassara sakamakon bayan mintuna 20.