【SUMMARY】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) uye cardiac Troponin I (cTnI) mapuroteni anobudiswa muropa mushure mekukuvara kwemwoyo. Myoglobin iheme-mapuroteni anowanzo kuwanikwa mumaskeletal uye mhasuru dzemwoyo ane huremu hwe17.8 kDa. Kana masero emhasuru akuvadzwa, Myoglobin inoburitswa muropa nekukurumidza nekuda kwekukura kwayo kudiki. Chiyero cheMyoglobin chinowedzera kuyera pamusoro pekutanga mukati me2-4 maawa post-infarct, ichikwira pa9-12 maawa, uye kudzokera kune yekutanga mukati me24-36 maawa. CK - MB ienzyme zvakare iripo mumhasuru yemwoyo, ine huremu hwe87.0 kDa. Creatine Kinase idimeric molecule yakaumbwa kubva kuma subunits maviri akadanwa se "M" uye "B", ayo anobatana kuita matatu akasiyana isoenzymes, CK-MM, CK-BB uye CK-MB. CK - MB ndiyo isoenzyme yeCreatine Kinase inonyanya kubatanidzwa mumetabolism yemhasuru yemwoyo tishu. Kuburitswa kweCK-MB muropa kunotevera MI kunogona kuonekwa mukati me3-8 maawa mushure mekutanga kwezviratidzo. Inokwira mukati me9 kusvika kumaawa makumi matatu, uye inodzokera kumazinga ekutanga mukati memaawa makumi mana nemasere kusvika makumi manomwe nemaviri. Cardiac Troponin I ipuroteni inowanikwa mumhasuru yemwoyo, ine molecular uremu hwe22.5 kDa. Troponin I chikamu chetatu subunit complex inoumbwa neTroponin T uye Troponin C. Pamwe chete netropomyosin, iyi structural complex inoumba chikamu chikuru chinogadzirisa calcium sensitive ATPase basa re actomyosin mu striated skeletal uye cardiac muscle. Mushure mokukuvadzwa kwemwoyo kunoitika, Troponin I inosunungurwa muropa 4 - 6 maawa mushure mekutanga kwemarwadzo. Nzira yekusunungurwa yeTroponin I yakafanana neCK - MB, asi nepo CK - MB mazinga achidzokera kune zvakajairika mushure memaawa 72, Troponin I inoramba yakasimudzwa kwemazuva 6 - 10, nokudaro ichipa hwindo refu rekuonekwa kwekukuvara kwemwoyo.

Iyo Myoglobin/CK-MB/Troponin I Combo Rapid Test Cassette (Ropa Rwose/Serum/Plasma) bvunzo iri nyore inoshandisa musanganiswa wezvimedu zvemasoja ekudzivirira chirwere uye mareagents ekutora kuti akwanise kuona Myoglobin, CK-MB uye yemwoyo Troponin I (cTnI) muropa rose. Chiyero chechidiki chekuonekwa i50ng/mL Myoglobin, 5ng/mL CK-MB uye 0.5ng/mL Troponin I.

 【MAZANO EKUSHANDISA】

Bvumira bvunzo kaseti, muenzaniso, buffer uye/kana zvidhiraivho kuti zvisvike tembiricha yemumba (15-30°C) isati yaedzwa.

1. Hunza homwe patembiricha yekamuri usati waivhura. Bvisa kaseti yebvunzo muhomwe yakavharwa woishandisa nekukasika.

2. Isa kaseti panzvimbo yakachena uye yakaenzana.

Kune Serum kana Plasma muenzaniso:

Bata chinodonhedza chakatwasuka woendesa madonhwe maviri e serum kana plasma (inenge 50µL) kumuenzaniso zvakanaka, wobva wawedzera 1 donhwe rebuffer (inenge 40µL), uye tanga iyo timer. Ona mufananidzo uri pasi apa.

YeVenipuncture Yese Ropa sampuli:

Bata chinodonhedza pasi uye woendesa madonhwe maviri eropa rose (inenge 50µL) kumuenzaniso zvakanaka, wobva wawedzera donhwe rimwe rebuffer (inenge 40µL), uye tanga chibatiso chenguva. Ona mufananidzo uri pasi apa.

YeFingerstick Yese Ropa muenzaniso:

· Kushandisa capillary chubhu: Zadza capillary chubhu uye kuendesa ingangoita 50µL yechigunwe chese ropa sampuli kumuenzaniso wetsime retest cassette, wobva wawedzera donhwe rimwe rebuffer (inenge 40µL) uye tanga chibatiso chenguva. Ona mufananidzo uri pasi apa.

· Kushandisa madonhwe ekurembera: Bvumira madonhwe maviri akarembera echigunwe chesese yeropa rose (inenge 50 µL) kuti awire munzvimbo yemuenzaniso yekaseti yekuyedza, wowedzera donho rimwe rebuffer (inenge 40µL) uye wotanga chibatiso chenguva. Ona mufananidzo uri pasi apa.

3. Mirira kuti mutsara wemavara (ma) uoneke. Verenga mhinduro pamaminetsi gumi. Usadudzire mhedzisiro mushure memaminitsi makumi maviri.