【TSEEM CEEB】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) thiab plawv Troponin I (cTnI) yog cov protein uas tso rau hauv cov hlab ntsha tom qab mob plawv. Myoglobin yog ib qho heme-protein ib txwm pom nyob rau hauv cov pob txha pob txha thiab lub plawv cov leeg nrog ib qho hnyav molecular ntawm 17.8 kDa. Thaum cov leeg nqaij puas lawm, Myoglobin tso tawm rau hauv cov ntshav sai vim nws qhov me me. Qib ntawm Myoglobin nce ntsuas siab tshaj qhov pib hauv 2 - 4 teev tom qab - infarct, peaking ntawm 9-12 teev, thiab rov qab mus rau hauv paus hauv 24-36 teev. CK-MB yog ib qho enzyme tseem muaj nyob rau hauv cov leeg mob plawv, nrog rau qhov hnyav molecular ntawm 87.0 kDa. Creatine Kinase yog ib qho dimeric molecule tsim los ntawm ob lub subunits uas yog "M" thiab "B", uas ua ke los ua peb yam isoenzymes, CK-MM, CK-BB thiab CK-MB. CK-MB yog lub isoenzyme ntawm Creatine Kinase feem ntau koom nrog hauv cov metabolism hauv cov leeg nqaij. Kev tso tawm CK-MB mus rau hauv cov ntshav tom qab MI tuaj yeem kuaj pom tsis pub dhau 3-8 teev tom qab pib cov tsos mob. Nws nce siab hauv 9 mus rau 30 teev, thiab rov qab mus rau theem pib hauv 48 txog 72 teev. Cardiac Troponin I yog cov protein uas pom nyob rau hauv cov leeg mob plawv, nrog rau qhov hnyav molecular ntawm 22.5 kDa. Troponin I yog ib feem ntawm peb lub subunit complex muaj xws li Troponin T thiab Troponin C. Nrog rau tropomyosin, cov txheej txheem no tsim cov ntsiab lus tseem ceeb uas tswj cov calcium rhiab heev ATPase kev ua ntawm actomyosin hauv striated skeletal thiab plawv cov leeg. Tom qab mob plawv tshwm sim, Troponin I raug tso rau hauv cov ntshav 4-6 teev tom qab pib mob. Cov qauv tso tawm ntawm Troponin I zoo ib yam li CK-MB, tab sis thaum CK-MB qib rov qab mus rau qhov qub tom qab 72 teev, Troponin Kuv tseem nce siab rau 6-10 hnub, yog li muab lub qhov rai ntev dua ntawm kev tshawb pom rau kev mob plawv.

Myoglobin / CK - MB / Troponin I Combo Rapid Test Cassette (Tag nrho Cov Ntshav / Ntshav / Ntshav / Plasma) yog ib qho kev sim yooj yim uas siv kev sib xyaw ua ke ntawm cov tshuaj tiv thaiv kab mob coated thiab ntes cov reagents kom pom zoo Myoglobin, CK-MB thiab plawv Troponin I (cTnI) hauv cov ntshav tag nrho. Qhov ntsuas qhov tsawg kawg nkaus yog 50ng / mL Myoglobin, 5ng / mL CK - MB thiab 0.5ng / mL Troponin I.

 【LUS QHIA RAU SIV】

Tso cai rau cov ntawv xeem, cov qauv, tsis thiab / lossis kev tswj kom ncav cuag chav sov (15 - 30 ° C) ua ntej kev sim.

1. Nqa lub hnab rau hauv chav sov ua ntej qhib nws. Tshem tawm cov ntawv xeem los ntawm lub hnab ntim khoom thiab siv nws sai li sai tau.

2. Muab lub cassette tso rau ntawm qhov chaw huv thiab theem.

Rau cov ntshav los yog Plasma cov qauv:

· Tuav lub dropper vertically thiab hloov 2 tee ntawm cov ntshav los yog ntshav (kwv yees li 50µL) rau hauv cov qauv zoo, tom qab ntawd ntxiv 1 poob ntawm qhov tsis sib xws (kwv yees li 40μL), thiab pib lub sijhawm. Saib daim duab hauv qab no.

Rau Venipuncture Whole Blood Specimen:

· Tuav lub dropper vertically thiab hloov 2 tee ntawm cov ntshav tag nrho (kwv yees li 50µL) rau hauv cov qauv zoo, tom qab ntawd ntxiv 1 tee ntawm qhov tsis zoo (kwv yees li 40µL), thiab pib lub sijhawm. Saib daim duab hauv qab no.

Rau Fingerstick Whole Blood specimen:

· Siv lub raj capillary: Sau lub raj capillary thiab hloov mus rau kwv yees li 50µL ntawm cov ntiv tes tag nrho cov ntshav mus rau hauv qhov dej ntawm qhov ntsuas ntsuas, tom qab ntawd ntxiv 1 tee ntawm qhov tsis muaj (kwv yees li 40µL) thiab pib lub sijhawm. Saib daim duab hauv qab no.

· Siv cov kais dai: Cia 2 dai tee ntawm cov ntiv tes tag nrho cov ntshav kuaj (kwv yees li 50 µL) kom poob rau hauv qhov chaw kuaj kab mob, tom qab ntawd ntxiv 1 tee ntawm qhov tsis zoo (kwv yees li 40µL) thiab pib lub sijhawm. Saib daim duab hauv qab no.

3. Tos kom pom cov kab uas muaj xim. Nyeem qhov tshwm sim ntawm 10 feeb. Tsis txhob txhais cov txiaj ntsig tom qab 20 feeb.