【CHIDULE】

Myoglobin (MYO), Creatine Kinase MB (CK-MB) ndi cardiac Troponin I (cTnI) ndi mapuloteni omwe amatulutsidwa m'magazi pambuyo pa kuvulala kwa mtima. Myoglobin ndi heme-mapuloteni omwe amapezeka m'minofu ya chigoba ndi yamtima ndi molekyulu yolemera 17.8 kDa. Maselo a minofu akawonongeka, Myoglobin imatulutsidwa m’magazi mofulumira chifukwa cha kukula kwake kochepa. Mulingo wa Myoglobin ukuwonjezeka moyezera pamwamba pa zoyambira mkati mwa 2-4 hours post-infarct, kufika pachimake pa 9-mawola 12, ndikubwereranso kumalo oyambira mkati mwa maola 24-36. CK-MB ndi puloteni yomwe imapezekanso mu minofu ya mtima, yokhala ndi kulemera kwa 87.0 kDa. Creatine Kinase ndi molekyulu ya dimeric yopangidwa kuchokera kumagulu awiri otchedwa "M" ndi "B", omwe amaphatikizana kupanga ma isoenzymes atatu, CK-MM, CK-BB ndi CK-MB. CK-MB ndi isoenzyme ya Creatine Kinase yomwe imakhudzidwa kwambiri ndi metabolism ya minofu ya mtima. Kutulutsidwa kwa CK-MB m’mwazi wotsatira MI kumatha kuzindikirika mkati mwa maola 3-8 chiyambireni zizindikiro. Imafika pachimake mkati mwa maola 9 mpaka 30, ndipo imabwereranso ku milingo yoyambira mkati mwa maola 48 mpaka 72. Cardiac Troponin I ndi puloteni yomwe imapezeka mu minofu ya mtima, yomwe imakhala ndi kulemera kwa 22.5 kDa. Troponin I ndi gawo la magawo atatu a subunit opangidwa ndi Troponin T ndi Troponin C. Pamodzi ndi tropomyosin, izi zimapangidwira chigawo chachikulu chomwe chimayang'anira calcium sensitive ATPase ntchito ya actomyosin mu striated chigoba ndi mtima minofu. Pambuyo pa kuvulala kwa mtima, Troponin I imatulutsidwa m'magazi 4 - maola 6 pambuyo poyambira kupweteka. Chitsanzo chomasulidwa cha Troponin I ndi chofanana ndi CK-MB, koma pamene CK-MB miyeso imabwereranso pambuyo pa maola a 72, Troponin I imakhalabe yapamwamba kwa 6 - masiku a 10, motero ndikupereka zenera lalitali lodziwikiratu kuvulala kwa mtima.

The Myoglobin/CK-MB/Troponin I Combo Rapid Test Cassette (Whole Blood/Serum/Plasma) ndi mayeso osavuta omwe amagwiritsa ntchito tinthu tating'ono tomwe timatitidwa ndi antibody ndi ma reagents kuti azindikire bwino Myoglobin, CK-MB ndi mtima Troponin I (cTnI) kapena plasma, seramu yonse. Mulingo wocheperako wozindikira ndi 50ng/mL Myoglobin, 5ng/mL CK-MB ndi 0.5ng/mL Troponin I.

 【MALANGIZO OGWIRITSA NTCHITO】

Lolani kuti makaseti oyesera, zitsanzo, zotchingira ndi/kapena zowongolera zifikire kutentha (15-30°C) musanayesedwe.

1. Bweretsani thumbalo kuti lizizizira bwino musanatsegule. Chotsani kaseti yoyesera m'thumba lomata ndikuigwiritsa ntchito posachedwa.

2. Ikani kaseti pamalo oyera komanso osalala.

Kwa zitsanzo za Seramu kapena Plasma:

Gwirani chotsitsacho molunjika ndikusamutsa madontho awiri a seramu kapena plasma (pafupifupi 50µL) pachitsanzocho bwino, kenaka yikani dontho limodzi la buffer (pafupifupi 40µL), ndikuyamba chowerengera. Onani chithunzi pansipa.

Kwa chitsanzo cha Magazi a Venipuncture:

Gwirani chotsitsacho molunjika ndikusamutsa madontho awiri amagazi athunthu (pafupifupi 50µL) kupita pachitsanzocho bwino, kenaka yikani dontho limodzi la buffer (pafupifupi 40µL), ndikuyamba chowerengera. Onani chithunzi pansipa.

Kwa chitsanzo cha Fingerstick Whole Blood:

Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndikusamutsa pafupifupi 50µL ya magazi athunthu a ndodo ya chala kupita pachitsime cha kaseti yoyesera, kenaka yikani dontho limodzi la buffer (pafupifupi 40µL) ndikuyamba chowerengera. Onani chithunzi pansipa.

Kugwiritsa ntchito madontho olendewera: Lolani madontho awiri olendewera a ndodo zonse zamagazi (pafupifupi 50 µL) kuti agwere mugawo lachitsanzo la makaseti oyesera, kenaka yikani dontho limodzi la buffer (pafupifupi 40µL) ndikuyamba chowerengera. Onani chithunzi pansipa.

3. Dikirani kuti mizere yachikuda iwonekere. Werengani zotsatira pa mphindi khumi. Osamasulira zotsatira pakatha mphindi 20.