【USHWANKATHELO】

I-Myoglobin (MYO), i-Creatine Kinase MB (CK-MB) kunye ne-cardiac Troponin I (cTnI) ziiprotheni ezikhutshwe kwigazi emva kokulimala kwentliziyo. I-Myoglobin yi-heme-iprotheni edla ngokufumaneka kwi-skeletal kunye ne-cardiac muscle enobunzima be-molecular ye-17.8 kDa. Xa iiseli zezihlunu zonakalisiwe, iMyoglobin ikhutshwa egazini ngokukhawuleza ngenxa yobukhulu bayo obuncinane. Inqanaba le-Myoglobin linyuka ngokulinganiswayo ngaphezu kwesiseko phakathi kwe-2-4 yeeyure zokuthumela-i-infarct, iphezulu kwi-9-12 iiyure, kwaye ibuyela kwisiseko phakathi kwe-24-36 iiyure. I-CK - MB i-enzyme ekhoyo kwakhona kwi-cardiac muscle, ene-molecular weight 87.0 kDa. I-Creatine Kinase yi-molecule ye-dimeric eyenziwe kwiinqununu ezimbini ezichongiweyo njenge "M" kunye ne "B", ezidibanisa ukwenza i-isoenzymes ezintathu ezahlukeneyo, CK - MM, CK - BB kunye ne-CK - MB. CK-MB yi-isoenzyme yeCreatine Kinase ebandakanyeka kakhulu kwi-metabolism yezicubu ze-cardiac muscle. Ukukhutshwa kwe-CK-MB egazini elilandela i-MI kunokubonwa kwisithuba seeyure ezi-3-8 emva kokuqala kweempawu. Ifikelela kwincopho phakathi kwe-9 ukuya kwiiyure ze-30, kwaye ibuyela kumanqanaba asisiseko kwiiyure ezingama-48 ukuya kwii-72. I-Cardiac Troponin I yiprotheni efumaneka kwi-cardiac muscle, ene-molecular weight 22.5 kDa. I-Troponin I yinxalenye ye-subunit complex equlethwe yi-Troponin T kunye ne-Troponin C. Kunye ne-tropomyosin, le ngqungquthela yesakhiwo yenza icandelo eliphambili elilawula umsebenzi we-ATPase we-calcium we-actomyosin kwi-striated skeletal and cardiac muscle. Emva kokulimala kwentliziyo kwenzeka, i-Troponin I ikhutshwa egazini 4 - iiyure ze-6 emva kokuqala kweentlungu. Ipatheni yokukhululwa ye-Troponin I ifana ne-CK - MB, kodwa ngelixa i-CK - amanqanaba e-MB abuyela ngokuqhelekileyo emva kweeyure ze-72, i-Troponin I ihlala iphakanyisiwe kwi-6 - iintsuku ze-10, ngaloo ndlela ibonelela ngefestile ende yokufumanisa ukulimala kwenhliziyo.

I-Myoglobin/CK-MB/Troponin I Combo Rapid Test Cassette (Igazi Elipheleleyo/iSerum/Plasma) luvavanyo olulula olusebenzisa indibaniselwano yamasuntswana e-antibody egqunywe nge-antibody kunye ne-reagents yokubamba ngokomgangatho we-Myoglobin, CK-MB kunye ne-cardiac Troponin I (cTnI) okanye i-plasma, i-plasma, i-serum. Ubuncinci benqanaba lokufumanisa ngu-50ng / mL Myoglobin, 5ng / mL CK-MB kunye ne-0.5ng/mL Troponin I.

 【IZIKHOKELO ZOKUSETYENZISWA】

Vumela ikhasethi yovavanyo, isampuli, isithinteli kunye/okanye izilawuli zifikelele kubushushu begumbi (15-30°C) phambi kovavanyo.

1. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuba usivule. Susa ikhasethi yovavanyo kwisingxobo esivaliweyo kwaye uyisebenzise ngokukhawuleza.

2. Beka ikhasethi kwindawo ecocekileyo kunye nenqanaba.

NgeSerum okanye isampuli yePlasma:

Bamba idropha ngokuthe nkqo kwaye udlulisele amathontsi ama-2 e-serum okanye iplasma (malunga ne-50µL) kumzekelo kakuhle, uze udibanise ithontsi enye ye-buffer (malunga ne-40µL), kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

KwiVenipuncture yeGazi elipheleleyo:

Bamba idropha ngokuthe nkqo kwaye udlulisele amathontsi ama-2 egazi elipheleleyo (malunga ne-50µL) kumzekelo kakuhle, uze udibanise ithontsi enye ye-buffer (malunga ne-40µL), kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

Ngomfanekiso weGazi Lomnwe weFingerstick:

· Ukusebenzisa ityhubhu ye-capillary: Gcwalisa ityhubhu ye-capillary kwaye ugqithisele malunga ne-50µL ye-fingerstick yegazi elipheleleyo kumzekelo wequla lekhasethi yovavanyo, uze wongeze ithontsi enye ye-buffer (malunga ne-40µL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

· Ukusebenzisa amathontsi ajingayo: Vumela amathontsi ama-2 ajingayo omfanekiso wegazi opheleleyo weminwe (malunga nama-50 µL) ukuba awele kwindawo yomzekelo wekhasethi yovavanyo, emva koko wongeze ithontsi enye ye-buffer (malunga ne-40µL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.

3. Lindela umgca onemibala ukuba uvele. Funda isiphumo ngemizuzu eyi-10. Musa ukutolika umphumo emva kwemizuzu engama-20.