【INCAMAKE】

Myoglobin (MYO), Creatine Kinase MB (CK - MB) na Cardiac Troponin I (cTnI) ni poroteyine zisohoka mu maraso nyuma yo gukomeretsa umutima. Myoglobin ni heme - proteine ​​isanzwe iboneka mumitsi ya skeletale na cardiac ifite uburemere bwa 17.8 kDa. Iyo ingirangingo z'imitsi zangiritse, Myoglobin irekurwa mumaraso byihuse kubera ubunini bwayo. Urwego rwa Myoglobin rwiyongera cyane hejuru ya baseline mugihe cyamasaha 2 - 4 post - infarct, igera kumasaha 9 - 12, hanyuma igasubira kumurongo mugihe cyamasaha 24 - 36. CK - MB ni enzyme nayo igaragara mumitsi yumutima, ifite uburemere bwa molekile ya 87.0 kDa. Creatine Kinase ni molekile ya dimeric ikozwe muri subunits ebyiri zagenwe nka "M" na "B", zihuza gukora isoenzymes eshatu zitandukanye, CK - MM, CK - BB na CK - MB. CK - MB ni isoenzyme ya Creatine Kinase igira uruhare runini muri metabolism yimitsi yumutima. Irekurwa rya CK - MB mumaraso akurikira MI irashobora kumenyekana mugihe cyamasaha 3 - 8 nyuma yuko ibimenyetso bitangiye. Igera hejuru mumasaha 9 kugeza 30, ikagaruka kurwego rwibanze mumasaha 48 kugeza 72. Cardiac Troponin I ni poroteyine iboneka mu mitsi yumutima, ifite uburemere bwa 22.5 kDa. Troponine I ni igice cyibice bitatu bya subunit bigizwe na Troponin T na Troponin C. Hamwe na tropomyosine, uru ruganda rwubatswe rugize igice cyingenzi kigenga calcium ikora ATPase yibikorwa bya actomyosine mumitsi ya skeletale na cardiac. Nyuma yo gukomeretsa k'umutima, Troponin I irekurwa mumaraso 4 - amasaha 6 nyuma yo gutangira ububabare. Uburyo bwo kurekura Troponin I busa na CK - MB, ariko mugihe urwego rwa CK - MB rusubira mubisanzwe nyuma yamasaha 72, Troponin I ikomeza kuzamuka muminsi 6 - 10, bityo igatanga idirishya rirerire ryo kumenya ibikomere byumutima.

Myoglobin / CK - MB / Troponin I Combo Yihuta Yipimishije Cassette (Amaraso Yuzuye / Serumu / Plasma) ni ikizamini cyoroshye gikoresha uruvangitirane rwa antibody ziteye kandi zifata reagent kugirango tumenye neza Myoglobin, CK - MB na Troponine I (cTnI) mumaraso yose, serumu cyangwa plasma. Urwego ntarengwa rwo kumenya ni 50ng / mL Myoglobin, 5ng / mL CK - MB na 0.5ng / mL Troponin I.

 【AMABWIRIZA YO GUKORESHA】

Emera cassette yikizamini, ingero, buffer na / cyangwa igenzura kugera kubushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha.

1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho cassette yikizamini mumufuka ufunze hanyuma uyikoreshe vuba bishoboka.

2. Shira cassette hejuru yisuku kandi iringaniye.

Kuri Serumu cyangwa Plasma urugero:

· Fata igitonyanga uhagarike hanyuma wohereze ibitonyanga 2 bya serumu cyangwa plasma (hafi 50µL) kurugero rwiza, hanyuma wongereho igitonyanga 1 cya buffer (hafi 40µL), hanyuma utangire igihe. Reba ingero zikurikira.

Kuri Venipuncture Amaraso Yuzuye:

· Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 2 byamaraso yose (hafi 50µL) kurugero rwiza, hanyuma wongereho igitonyanga 1 cya buffer (hafi 40µL), hanyuma utangire igihe. Reba ingero zikurikira.

Kuri Fingerstick Amaraso Yuzuye:

· Gukoresha umuyoboro wa capillary: Uzuza umuyoboro wa capillary hanyuma wohereze hafi 50µL yintoki zintoki zose zamaraso kurugero rwiza rwa cassette yipimishije, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40µL) hanyuma utangire igihe. Reba ingero zikurikira.

· Gukoresha ibitonyanga bimanikwa: Emerera ibitonyanga 2 bimanitse byerekana urutoki rwamaraso yose (hafi 50 µL) kugwa mumwanya wikigereranyo cya cassette, hanyuma ongeramo igitonyanga 1 cya buffer (hafi 40µL) hanyuma utangire igihe. Reba ingero zikurikira.

3. Tegereza umurongo (amabara) ugaragara. Soma ibisubizo muminota 10. Ntugasobanure ibisubizo nyuma yiminota 20.