【ZVIRI KUSHANDISA】 

Clostridium difficile ibhakitiriya reanaerobic rinoshanda sechirwere chetachiona: chinokura muura kana maruva akajairika akachinjwa nekurapwa nemishonga inorwisa mabhakitiriya. Toxinogenic strains yeClostridium difficile inokonzeresa kutapukirwa kubva ku milddiarrhea kusvika kupseudomembranous colitis, zvinogona kutungamira kurufu. Chirwere chinokonzerwa nehupfu huviri hunogadzirwa netoxinogenic strains yeC.difficile: Toxin A (tishu-inokuvadza enterotoxin) uye Muchetura B (cytotoxin). Mamwe marudzi anoburitsa zvese chepfu A neB, mamwe anoburitsa Toxin B chete. Basa rinogona kuitwa rechitatu (binary) chepfu mukupathogenicity ichiri kunetsana. Kushandiswa kweGlutamate Dehydrogenase (GDH) se antigen marker yeC.difficile proliferation kwakaratidzwa kuva kunoshanda zvikuru nekuti marudzi ose anobudisa huwandu hwakawanda hweenzyme iyi. Clostridium K-SeT inobvumira kuonekwa kweC.difficile's GDH mumuenzaniso wezvituru. Sampuli dzine Clostridium K-SeT yakanaka dzinofanira kuongororwa kuti dzionekwe kana utachiona hune huturu.

【SUMMARY】  

Clostridia (nhengo dzegenus Clostridium) ianaerobic, motile bacteria, rinowanikwa pose pose, uye kunyanya kuwanda muvhu. Pasi pemaikorosikopu, anoonekwa akareba, asina kuenzana (kazhinji drumstick- kana spindle-shaped) maseru ane bundu kumagumo awo. Pasi peGram staining, C. difficile cells ane Gram-positive uye anoratidza kukura kwakanyanya paagar yeropa pakudziya kwemuviri wemunhu pasina oxygen. Kana ikamanikidzwa, bhakitiriya inoburitsa spores inokwanisa kushivirira mamiriro ezvinhu asingakwanise kushivirirwa nebhakitiriya rinoshanda. C. difficile inogona kugadzika mucolon yemunhu; iripo mu2–5% yevanhu vakuru. Dzimwe nguva kurapa kwemishonga inorwisa mabhakitiriya kwezvirwere zvakasiyana-siyana kune kwakaipa kukanganisa kuenzana kwezvinomera ura, zvinozoitika kuti C. difficile inogona kutonga, zvichikonzera utachiona hweClostridium difficile.

【MAZANO EKUSHANDISA】  

Bvumira mudziyo wekuyedza, sampu yekuyedza uye buffer kuti ienderane netembiricha yemumba (15-30°C) isati yaedzwa. Ndokumbira utarise kadhi remaitiro mune iyi kit. Vhura homwe wobvisa mudziyo. Kana yangovhurwa, mhanyisa bvunzo nekukurumidza. Ratidza zita remurwere kana nhamba yemuenzaniso pamudziyo (mudziyo mumwe pasample)

1. Vhura iyo FSS chubhu uye shandisa sikururu kuunganidza stool sample (A). The dilution reshiyo inofanira kunge iri 4% w/v. Chenjerera kuti usatora yakawandisa (B1) kana kuti specimen (B2) yakawandisa. Kumasamples emvura kana semi-emvura, pipette 80 μL yesampuli uchishandisa micropipette (isina kupihwa) muFSS vial.

2. Isai screw muFSS uye simbisa chivharo. Vortex gadziriro ye homogenize (C). Iyo yese stool samples inofanirwa kusungirirwa mune mhinduro.

3. Bvisa poindi yekepisi (D) wobva waisa madonhwe mana esampuli yakanyungudutswa mu tsime remuenzaniso remudziyo sezvinoratidzwa pazasi. Kuti uve nechokwadi chekutumira kwakakodzera, FSS vial inofanira kubatwa yakatwasuka 4. Siya kuti uite maminetsi gumi nemashanu. Mibairo inocherechedzwa pahwindo rekuverenga.