【Ezubere iji】 

Clostridium difficile bụ nje bacteria anaerobic na-eme dị ka ihe na-akpata ohere: ọ na-etolite n'ime eriri afọ mgbe ejiri ọgwụ nje mee ihe gbanwere ahịhịa nkịtị. Ụdị toxinogenic nke Clostridium difficile na-ebute ọrịa sitere na milddiarrhea ruo pseudomembranous colitis, nwere ike ibute ọnwụ. A na-ebute ọrịa site na nsị abụọ nke ụdị toxinogenic nke C.difficile na-emepụta: Toxin A (anụ ahụ - enterotoxin na-emebi) na Toxin B (cytotoxin). Ụfọdụ ụdịdị na-emepụta ma nsi A na B, ụfọdụ ndị ọzọ na-emepụta naanị Toxin B. A ka na-arụrịta ụka maka ọrụ nwere ike nke atọ (ọnụọgụ abụọ) nsí n'ọrịa nje. Ojiji Glutamate Dehydrogenase (GDH) dị ka antigen nrịbama nke C.difficile proliferation na-adị irè nke ukwuu n'ihi na ụdịdị niile na-emepụta nnukwu enzyme a. Clostridium K-SeT na-enye ohere ịchọpụta kpọmkwem C.difficile's GDH n'ụdị stool. A ga-enyocha ihe nlele ndị nwere ezigbo Clostridium K-SeT nsonaazụ iji nwalee maka nsi nke nje ahụ.

【Nchịkọta】  

Clostridia (ndị otu genus Clostridium) bụ anaerobic, nje bacteria na-agagharị agagharị, dị ebe niile n'okike, yana ọkachasị n'ime ala. N'okpuru microscope, ha na-apụta ogologo oge, na-adịghị agbanwe agbanwe (na-abụkarị drumstick- ma ọ bụ spindle- nwere ọdịdị) mkpụrụ ndụ nwere mpụta na nsọtụ ha. N'okpuru ntụpọ Gram, mkpụrụ ndụ C. difficile bụ Gram - dị mma ma na-egosi uto kacha mma na agar ọbara na okpomọkụ nke ahụ mmadụ na enweghị oxygen. Mgbe nrụgide siri ike, nje bacteria na-emepụta spores nke nwere ike idi oke ọnọdụ nke nje bacteria na-arụ ọrụ enweghị ike ịnagide. C. nwere ike ịpụta ihe siri ike na eriri afọ mmadụ; ọ dị na 2–5% nke ndị okenye. Mgbe ụfọdụ ọgwụgwọ ọgwụ nje maka ọrịa dị iche iche na-enwe mmetụta ọjọọ nke na-akpaghasị nguzozi nkịtị nke eriri afọ, n'ọnọdụ nke C. difficile nwere ike na-achịkwa n'ụzọ kwesịrị ekwesị, na-ebute ọrịa Clostridium siri ike.

【Ntuziaka maka ojiji】  

Kwe ka ngwaọrụ nnwale ahụ, nlele nlele na nchekwa ka ọ dakọtara na ọnụ ụlọ (15-30°C) tupu nnwale. Biko rụtụ aka na kaadị usoro na ngwa a. Mepee obere akpa ahụ wee wepụ ngwaọrụ ahụ. Ozugbo emepere, gbaa ule ozugbo. gosi aha onye ọrịa ma ọ bụ nọmba nlere anya na ngwaọrụ ahụ (otu ngwaọrụ kwa sample)

1. Mepee tube FSS ma jiri nkedo na-anakọta ihe nlele stool (A). Oke ntughari ga-abụrịrị ihe dịka 4% w/v. Kpachara anya ka ị ghara iburu oke (B1) ma ọ bụ obere specimen (B2). Maka nlere mmiri mmiri ma ọ bụ ọkara-ihe nlele mmiri, pipette 80 μL nke ihe nlele na-eji micropipette (anaghị enye ya) n'ime vial FSS.

2. Tinye ịghasa n'ime FSS ma mee ka okpu sie ike. Vortex nkwadebe ka homogenize (C). A ghaghị ịkwụsịtụ ihe nlele stool niile n'ime ngwọta.

3. Gbajie ebe okpu (D) wee fesaa ntụle 4 nke ntụsara ahụ n'ime ihe nlele nke ngwaọrụ dị ka egosiri n'okpuru. Iji jide n'aka na nnyefe kwesịrị ekwesị, FSS vial ga-ejiderịrị na kwụ ọtọ 4. Hapụ ka ọ meghachi omume maka nkeji iri na ise. A na-ahụ nsonaazụ ya na windo ọgụgụ.