【UKORESHEJWE】 

Clostridium difficile ni bagiteri ya anaerobic ikora nka virusi itera amahirwe: ikura mu mara mugihe ibimera bisanzwe byahinduwe no kuvura antibiyotike. Uburozi bwa Toxinogenic bwa Clostridium difficile butera kwandura indwara zidakira zanduye na kolite ya pseudomembranous colitis, bishobora gutera urupfu. Indwara ziterwa nuburozi bubiri buterwa nuburozi bwa C.difficile: Toxin A (tissue - yangiza enterotoxine) na Toxin B (cytotoxine). Ubwoko bumwe butanga uburozi A na B, ubundi butanga Uburozi B gusa. Uruhare rushoboka rwuburozi bwa gatatu (binary) mubitera indwara biracyaganirwaho. Gukoresha Glutamate Dehydrogenase (GDH) nk'ikimenyetso cya antigen yo gukwirakwiza C.difficile byagaragaye ko ari ingirakamaro cyane kuko amoko yose atanga umusaruro mwinshi w'iyi misemburo. Clostridium K - SeT yemerera gutahura neza GDH ya C.difficile muburyo bw'intebe. Ingero zifite Clostridium nziza K - Ibisubizo bya SeT bigomba gukorwaho ubushakashatsi kugirango hamenyekane uburozi bwa bagiteri.

【INCAMAKE】  

Clostridia (abagize ubwoko bwa Clostridium) ni anaerobic, bacteri motile, igaragara hose muri kamere, kandi yiganje cyane mubutaka. Munsi ya microscope, zigaragara nkizirebire, zidasanzwe (akenshi ingoma - Munsi ya Gram irangi, C. diffile selile ni Gram - nziza kandi yerekana imikurire myiza kuri agar yamaraso kubushyuhe bwumubiri wumuntu mugihe ogisijeni idahari. Iyo uhangayitse, bagiteri itanga intanga ngabo zishobora kwihanganira ibihe bikabije bagiteri zikora zidashobora kwihanganira. C. diffile irashobora gushirwaho mumurongo wabantu; irahari muri 2-5% byabaturage bakuze. Rimwe na rimwe, antibiyotike ivura indwara zitandukanye igira ingaruka mbi zo guhungabanya uburinganire busanzwe bw’ibimera byo mu nda, icyo gihe C. diffile irashobora kwiganza mu buryo bworoshye, bigatuma Clostridium difficile yandura.

【AMABWIRIZA YO GUKORESHA】  

Emera igikoresho cyo kwipimisha, icyitegererezo hamwe na buffer kuringaniza ubushyuhe bwicyumba (15 - 30 ° C) mbere yo kwipimisha. Nyamuneka reba ikarita yuburyo bukoreshwa muri iki gitabo. Fungura umufuka ukureho igikoresho. Bimaze gukingurwa, kora ikizamini ako kanya. Erekana izina ry'umurwayi cyangwa nimero y'icyitegererezo ku gikoresho (igikoresho kimwe kuri sample)

1. Fungura umuyoboro wa FSS hanyuma ukoreshe umugozi wo gukusanya icyitegererezo cy'intebe (A). Ikigereranyo cyo kugabanuka kigomba kuba hafi 4% w / v. Witondere kudafata byinshi (B1) cyangwa urugero ruto cyane (B2). Kubisukari cyangwa igice - icyitegererezo cyamazi, pipette 80 μL yicyitegererezo ukoresheje micropipette (idatanzwe) mumashanyarazi ya FSS.

2. Shyiramo umugozi muri FSS hanyuma ukomere ingofero. Vortex imyiteguro yo guhuza ibitsina (C). Icyitegererezo cyose cyintebe kigomba guhagarikwa mugisubizo.

3. Kuraho ingingo yumutwe (D) hanyuma utange ibitonyanga 4 byurugero rwicyitegererezo mu iriba ryicyitegererezo nkuko bigaragara hano hepfo. Kugirango wizere neza ko itangwa neza, vial ya FSS igomba kuba ihagaritse 4. Kureka kubyitwaramo muminota 15. Ibisubizo bigaragara mu idirishya ryo gusoma.