【UKUSETSHENZISWA OKUHLOSIWE】 

I-Clostridium difficile igciwane le-anaerobic elisebenza njenge-pathogen engosomathuba: likhula emathunjini lapho isitshalo esivamile sishintshiwe ngokwelashwa ngemithi elwa namagciwane. Izinhlobo ze-toxinogenic ze-Clostridium difficile zidala izifo ezisuka esihudo esincane kuya ku-pseudomembranous colitis, okungase kuholele ekufeni. Isifo sibangelwa ubuthi obubili obukhiqizwa izinhlobo ze-toxinogenic ze-C.difficile: Ubuthi A (izicubu-ezilimazayo i-enterotoxin) kanye ne-Toxin B (cytotoxin). Ezinye izinhlobo zikhiqiza kokubili ubuthi A no-B, ezinye ezinye zikhiqiza i-Toxin B kuphela. Indima engaba khona ye-toxin yesithathu (inambambili) ku-pathogenicity kusaxoxwa ngayo. Ukusetshenziswa kwe-Glutamate Dehydrogenase (GDH) njenge-antigen marker ye-C.difficile proliferation kuboniswe ukuthi kusebenza kahle ngoba zonke izinhlobo zikhiqiza inani eliphezulu lale enzyme. I-Clostridium K-SeT ivumela ukutholwa okuqondile kwe-GDH ye-C.difficile kusifanekiso sezihlalo. Amasampuli anomphumela omuhle we- Clostridium K-SeT kufanele aphenywe kabanzi ukuze kuhlolwe ubuthi bamagciwane.

【ISIFINYEZO】  

I-Clostridia (amalungu ohlobo lwe-Clostridium) ayi-anaerobic, amagciwane anyakazayo, etholakala yonke indawo ngemvelo, futhi avame kakhulu enhlabathini. Ngaphansi kwe-microscope, avela amaseli amade, angajwayelekile (ngokuvamile e-drumstick- noma anomumo we-spindle-) aneqhuqhuva ekugcineni kwawo ukuphela. Ngaphansi kwe-Gram staining, amaseli e-C. difficile ane-Gram-positive futhi abonisa ukukhula okuphezulu ku-agar yegazi emazingeni okushisa omzimba womuntu lapho ungekho umoya-mpilo. Uma ecindezelekile, amagciwane akhiqiza izinhlamvu ezikwazi ukubekezelela izimo ezidlulele amagciwane asebenzayo angakwazi ukuzibekezelela. C. difficile ingase isungulwe kukholoni yomuntu; ikhona ku-2–5% wabantu abadala. Ngezinye izikhathi ukwelashwa ngama-antibiotic kwezifo ezihlukahlukene kuba nomthelela omubi wokuphazamisa ibhalansi evamile yezitshalo zamathumbu, lapho kwenzeka ukuthi i-C. difficile ilawuleke ngokwethuba, ibangele ukutheleleka kwe-Clostridium difficile.

【IZINDLELA ZOKUSEBENZISA】  

Vumela idivayisi yokuhlola, isampula yokuhlola nebhafa ukuthi ilingane nezinga lokushisa legumbi (15-30°C) ngaphambi kokuhlolwa. Sicela ubheke ikhadi lenqubo kule kit. Vula isikhwama bese ukhipha idivayisi. Uma isivuliwe, yenza ukuhlola ngokushesha. Bonisa igama lesiguli noma inombolo yesifanekiso kudivayisi (idivayisi eyodwa ngesampula ngayinye)

1. Vula ishubhu le-FSS bese usebenzisa isikulufu ukuze uqoqe isampula yesitulo (A). Isilinganiso se-dilution kufanele sibe cishe ngu-4% w/v. Qaphela ukuthi ungathathi kakhulu (B1) noma i-specimen (B2) encane kakhulu. Kumasampula awuketshezi noma awuncane-owuketshezi, i-pipette 80 μL yesampula kusetshenziswa i-micropipette (akunikeziwe) kuvial ye-FSS.

2. Faka isikulufu ku-FSS bese uqinisa ikepisi. Vortex ukulungiselela ukuze homogenize (C). Isampula yonke yesitulo kufanele imiswe okwesikhashana kusixazululo.

3. Hlukanisa iphoyinti lekepisi (D) bese ukhipha amathonsi angu-4 esampuli exutshiwe emthonjeni wesampula wedivayisi njengoba kuboniswe ngezansi. Ukuqinisekisa ukulethwa okufanele, i-FSS vial kumele ibanjwe iqonde 4. Yiyeke ukuze usabele imizuzu engu-15. Imiphumela ibonwa efasiteleni lokufunda.