【ZOYENERA KUGWIRITSA NTCHITO】 

Clostridium difficile ndi bakiteriya wa anaerobic omwe amagwira ntchito ngati tizilombo toyambitsa matenda: amamera m'matumbo pamene zomera zomwe zili bwino zasinthidwa pothandizidwa ndi maantibayotiki. Mitundu ya Toxinogenic ya Clostridium difficile imayambitsa matenda kuchokera ku matenda otsekula m'mimba mpaka pseudomembranous colitis, omwe amatha kupha. Matenda amayamba ndi poizoni awiri opangidwa ndi mitundu ya toxinogenic ya C.difficile: Toxin A (minofu-owononga enterotoxin) ndi Toxin B (cytotoxin). Mitundu ina imatulutsa zonse poizoni A ndi B, ina imatulutsa Poizoni B kokha. Kuthekera kwa kawopsedwe kachitatu (koyipana) pa pathogenicity kumatsutsanabe. Kugwiritsa ntchito Glutamate Dehydrogenase (GDH) monga antigen marker ya C.difficile proliferation kwasonyezedwa kuti n'kothandiza kwambiri chifukwa mitundu yonse imatulutsa kuchuluka kwa enzyme imeneyi. Clostridium K-SeT imalola kuzindikirika kwachindunji kwa C.difficile's GDH mu chitsanzo cha ndowe. Zitsanzo zokhala ndi zotsatira zabwino za Clostridium K-SeT ziyenera kufufuzidwanso kuti muwone ngati mabakiteriya ali ndi poizoni.

【CHIDULE】  

Clostridia (mamembala a Clostridium) ndi anaerobic, mabakiteriya oyenda, amapezeka paliponse, ndipo amapezeka kwambiri m'nthaka. Pansi pa maikulosikopu, amawoneka ngati maselo aatali, osasamba (nthawi zambiri ngati drumstick- kapena spindle-shaped) maselo okhala ndi chotupa kumapeto kwake. Pansi pa Gram staining, ma cell a C. difficile ali ndi Gram-positive ndipo amawonetsa kukula bwino kwa agar wamagazi pakutentha kwa thupi la munthu pakapanda mpweya. Akapanikizika, mabakiteriyawa amapanga spores omwe amatha kupirira zinthu zoipitsitsa zomwe mabakiteriya omwe akugwira ntchito sangathe kupirira. C. difficile akhoza kukhazikika m'matumbo a munthu; imapezeka mu 2–5% ya anthu akuluakulu. Nthawi zina maantibayotiki a matenda osiyanasiyana amatha kukhala ndi zotsatira zoyipa zosokoneza kukhazikika kwa m'matumbo, motero C. difficile amatha kulamulira mwamwayi, zomwe zimayambitsa matenda a Clostridium difficile.

【MALANGIZO OGWIRITSA NTCHITO】  

Lolani chipangizo choyesera, zitsanzo zoyesera ndi buffer kuti zigwirizane ndi kutentha kwa chipinda (15-30°C) zisanachitike. Chonde onani khadi lakachitidwe lomwe lili pagululi. Tsegulani thumba ndikuchotsa chipangizocho. Mukatsegulidwa, yesani kuyesa nthawi yomweyo. Onetsani dzina la wodwalayo kapena nambala yake yachitsanzo pachipangizocho (chipangizo chimodzi pa chitsanzo chilichonse)

1. Tsegulani chubu la FSS ndikugwiritsa ntchito screw kuti mutenge chitsanzo cha ndowe (A). Dilution ayenera kukhala pafupifupi 4% w/v. Samalani kuti musatenge zambiri (B1) kapena chitsanzo chaching'ono (B2). Kwa zitsanzo zamadzimadzi kapena theka-zamadzimadzi, pipette 80 μL ya zitsanzo pogwiritsa ntchito micropipette (osaperekedwa) mu vial ya FSS.

2. Lowetsani wononga mu FSS ndikumangitsa kapu. Vortex kukonzekera ku homogenize (C). Chitsanzo chonse cha chopondapo chiyenera kuyimitsidwa mu yankho.

3. Chotsani kapu (D) ndikutaya madontho 4 a zitsanzo zosungunuka mu chitsime chachitsanzo cha chipangizochi monga momwe zasonyezedwera pansipa. Kuti mutsimikizire kutumizidwa koyenera, FSS vial iyenera kusungidwa molunjika 4. Siyani kuti muyankhe kwa mphindi 15. Zotsatira zimawonedwa pawindo lowerengera.