【ZVIRI KUSHANDISA】

The Cardiac Troponin I Rapid Test Device (Colloidal Gold) inokurumidza chromatographic immunoassay yekuonekwa kwehunhu hwemwoyo wemunhu Troponin I muropa rose, serum kana plasma seyamuro mukuongororwa kwemyocardial infarction (MI).

【SUMMARY】

Cardiac Troponin I (cTnI) ipuroteni inowanikwa mumhasuru yemwoyo ine molecular uremu hwe22.5 kDa.1 Troponin I chikamu chetatu subunit complex inosanganisira Troponin T uye Troponin C. Pamwe chete netropomyosin, iyi structural complex inoumba chikamu chikuru chinogadzirisa calcium activrisiti yeAATPase muscleac-sensitive muscle basa. 2 Mushure mekukuvara kwemwoyo kunoitika, Troponin I inosunungurwa muropa 4-6 maawa mushure mekutanga kwemarwadzo. Nzira yekusunungurwa ye cTnI yakafanana neCK - MB, asi nepo CK - MB mazinga achidzokera kune zvakajairika mushure memaawa 72, Troponin I inoramba yakasimudzwa kwemazuva 6 - 10, nokudaro ichipa hwindo refu rekuonekwa kwekukuvara kwemwoyo. Iyo yakanyanya kujeka yezviyero zvecTnI zvekuzivikanwa kwekukuvara kwemyocardial kwakaratidzwa mumamiriro ezvinhu akadai senguva yeperioperative, mushure mekumhanya kwemarathon, uye blunt chest trauma. 3 cTnI kusunungurwa kwakanyorwawo mumamiriro emwoyo kunze kweacute myocardial infarction (AMI) yakadai seangina isina kugadzikana, congestive heart failure, uye ischemic kukuvara nekuda kwekuvhiyiwa kwecoronary artery bypass. 4 Nemhaka yehupamhi hwepamusoro uye kunzwisiswa muhutano hwemyocardial, Troponin I ichangobva kuva iyo inonyanya kufarirwa biomarker ye myocardial infarction.5

Iyo Cardiac Troponin I Rapid Test Device(Colloidal Goridhe) muyedzo uri nyore unoshandisa musanganiswa weanti- cTnI antibody yakavharwa zvimedu uye reagent yekubata kuona cTnI muropa rakazara, serum kana plasma. Chiyero chechidiki chekuonekwa i0.5ng/mL.

【NZIRA YEKUSHANDISA】

Bvumira bvunzo, muenzaniso, buffer uye/kana zvidhinha kuti zvisvike tembiricha yemumba (15-30℃) vasati vaedzwa.

1.Hunza homwe kukamuri tembiricha usati waivhura.Bvisa bvunzo Chishandiso kubva muhomwe yakavharwa uye uishandise nekukurumidza sezvinobvira.

2.Isa Chidimbu panzvimbo yakachena uye yepamusoro.

ForSerum kana Plasma muenzaniso:

Bata dropper vertically uye kuendesa2 madonhwe e serum kana plasma (anenge makumi mashanuµL)kuenda kunzvimbo yemuenzaniso, ipapo wedzera 1 madonhwe e-buffer(inenge 40µL), uye tanga iyo timer. Ona mufananidzo uri pasi apa.

ForVenipuncture Ropa rose muenzaniso:

Bata dropper vertically uye kuendesa3 madonhwe eropa rose (inenge75µL) kunzvimbo yemuenzaniso, ipapowedzera 1 madonhwe e-buffer (inenge40µL), uye tanga chibatiso chenguva.Ona mufananidzo uri pazasi.

ForFingerstick Yese Ropa Muenzaniso:

Kushandisa capillary chubhu: Zadza capillary chubhuuye kutamisa angangoita 75µL of chigunwe cheropa rose muenzanisokune iyo specimen nzvimbo yekuyedza Chidimbu, ipapo wedzera 1 donho rebuffer (inenge 40µL) uye tanga chibatiso chenguva.Ona mufananidzo uri pazasi.

Kushandisa madonhwe ekurembera: BvumiraMadonhwe matatu akarembera echigunwe cheropa rose muenzaniso (inenge 75µL) kuwira munzvimbo yemuenzaniso yeyedzo Chishandiso, ipapowedzera 1 donho re buffer (inenge 40µL) uye tanga chibatiso chenguva.Ona mufananidzo uri pazasi.

3. Mirira kuti mutsara wemavara (ma) uoneke.Verenga zvawanikwa pamaminitsi gumi.Usadudzire mhinduro mushure memaminitsi makumi maviri.