【UKUSETSHENZISWA OKUHLOSIWE】

I-Cardiac Troponin I Rapid Test Device (Colloidal Gold) iwukuhlolwa kwe-chromatographic okusheshayo ukuze kutholwe ikhwalithi yenhliziyo yomuntu i-Troponin I egazini lonke, i-serum noma i-plasma njengosizo ekuxilongweni kwe-myocardial infarction (MI).

【ISIFINYEZO】

I-Cardiac Troponin I (cTnI) iphrotheni etholakala kusisipha senhliziyo enesisindo sengqamuzana esingu-22.5 kDa.1 I-Troponin I iyingxenye ye-subunit complex ehlanganisa i-Troponin T ne-Troponin C. Kanye ne-tropomyosin, le nkimbinkimbi yesakhiwo yakha ingxenye eyinhloko elawula umsebenzi we-calcium actriated we-ATPase ye-calcium e-accleacion muscle ye-ATPaseackeletal muscle sensitive. 2 Ngemva kokulimala kwenhliziyo kwenzeka, i-Troponin I ikhishwa egazini 4-6 amahora ngemva kokuqala kobuhlungu. Iphethini yokukhululwa ye-cTnI ifana ne-CK-MB, kodwa kuyilapho amazinga e-CK-MB ebuyela kokujwayelekile ngemva kwamahora angu-72, i-Troponin I ihlala iphakeme izinsuku ezingu-6-10, ngaleyo ndlela inikeze iwindi elide lokutholwa kokulimala kwenhliziyo. Ukucaciswa okuphezulu kwezilinganiso ze-cTnI zokuhlonza ukulimala kwe-myocardial kuboniswe ezimeni ezifana nenkathi ye-perioperative, ngemva kokugijima kwe-marathon, kanye nokulimala kwesifuba okufiphele. 3 Ukukhishwa kwe-cTnI kuphinde kwabhalwa ezimweni zenhliziyo ngaphandle kwe-acute myocardial infarction (AMI) njenge-angina engazinzile, ukwehluleka kwenhliziyo eqinile, nokulimala kwe-ischemic ngenxa yokuhlinzwa kwe-coronary artery bypass. I-4 Ngenxa yokucaciswa kwayo okuphezulu nokuzwela kwezicubu ze-myocardial, i-Troponin I isanda kuba yi-biomarker ekhethwa kakhulu ye-myocardial infarction.5

I-Cardiac Troponin I Rapid Test Device(i-Colloidal Gold) iwukuhlola okulula okusebenzisa inhlanganisela yezinhlayiya ezimboze i-anti-cTnI antibody kanye ne-reagent yokuthwebula ukuze kutholwe i-cTnI egazini lonke, i-serum noma i-plasma. Izinga eliphansi lokutholwa ngu-0.5ng/mL.

【ISIQONDISO SOKUSETSHENZISWA】

Vumela ukuhlola, i-specimen, ibhafa kanye/noma izilawuli ukuthi zifinyelele izinga lokushisa legumbi (15-30℃) ngaphambi kokuhlolwa.

1.Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Susa idivayisi yokuhlola esikhwameni esivaliwe futhi uyisebenzise ngokushesha ngangokunokwenzeka.

2.Beka Idivayisi endaweni ehlanzekile nesezingeni.

NgobaIsifanekiso seSerum noma sePlasma:

Bamba i-dropper ime mpo bese uyidluliselaAmaconsi ama-2 we-serum noma i-plasma (cishe ama-50µL)endaweni yesifanekiso, ke engeza amaconsi angu-1 ebhafa(cishe 40µL), bese uqala isibali sikhathi. Bheka umfanekiso ngezansi.

NgobaI-Venipuncture Igazi Eliphelele:

Bamba i-dropper ime mpo bese uyidluliselaAmaconsi ama-3 egazi eliphelele (cishe75µL) endaweni yesifanekiso, beseengeza amaconsi angu-1 ebhafa (cishe40µL), bese uqala isibali sikhathi.Bona umfanekiso ngezansi.

NgobaIsifanekiso seFingerstick Whole Blood:

Ukusebenzisa ithubhu le-capillary: Gcwalisa ishubhu le-capillaryfuthi udlulise cishe ama-75µL of umunwe wegazi elipheleleendaweni yesifanekiso sedivayisi yokuhlola, bese engeza ithonsi elingu-1 lebhafa (cishe u-40µL) bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

Ukusebenzisa amaconsi alengayo:VumelaAmaconsi ama-3 alengayo wezinti zegazi eliphelele (cishe 75µL) ukuwela endaweni yesifanekiso sedivayisi yokuhlola, beseengeza 1 iconsi ibhafa (cishe 40µL) bese uqala isibali sikhathi.Bheka umfanekiso ngezansi.

3. Linda kuze kuvele i(ama)layini anombala.Funda imiphumela ngemizuzu eyi-10.Ungahumushi umphumela ngemuva kwemizuzu engama-20.