【Awọn ilana Ilana】

2019-nCOV IgM Antibody Assay Reagent Kit (CMIA) jẹ meji-igbesẹ immunoassay nipa lilo imọ-ẹrọ kemiluminescence patikulu oofa. O le pinnu ni deede wiwa awọn egboogi-2019-nCOVIgM awọn aporo inu omi ara eniyan tabi pilasima.

Lakoko ifura naa, igbesẹ akọkọ jẹ abuda amuaradagba nipa didapọ ayẹwo pẹlu awọn patikulu paramagnetic to lagbara ti a bo pẹlu antijeni amuaradagba iparun 2019-nCoV. Lẹhin fifọ, alkaline phosphataselabeled murine anti- antibody IgM monoclonal eda eniyan ni a fi kun ni igbesẹ keji lati ṣe patikulu oofa kan-nucleoprotein_anti-2019-nCoV-IgM murine-anti-IgM-ALP-àdàpọ̀ àbájáde enzyme. Lẹhin iyipo iwẹ miiran, sobusitireti ti phosphatase ipilẹ ti wa ni afikun si adalu ifaseyin. Abajade chemiluminescence esi lẹhinna ni iwọn ni awọn ẹya ina ibatan (RLU). Idojukọ atako -2019-nCoV - antibody IgM ninu ayẹwo jẹ iwọn si iye awọn ẹya ina ibatan (RLU) ti adalu. Nitorinaa abajade le ṣee lo lati rii wiwa IgM ninu ayẹwo ati iranlọwọ lati pinnu boya alaisan naa ti ni akoran nipasẹ ọlọjẹ 2019-nCoV.