【Izimiso Zenqubo】

I-2019-nCOV IgM Antibody Assay Reagent Kit (CMIA) iyizinyathelo ezimbili - ukuhlolwa kokuzivikela komzimba kusetshenziswa ubuchwepheshe bezinhlayiyana kazibuthe chemiluminescence. Ingakwazi ukucacisa ngokunembile ukuba khona kwamasosha omzimba alwa -2019-nCOVIgM ku-serum yomuntu noma ku-plasma.

Ngesikhathi sokusabela, isinyathelo sokuqala ukubopha amaprotheni ngokuxuba isampula nezinhlayiya eziqinile ze-paramagnetic ezimbozwe nge-antigen ye-nuclear protein 2019-nCoV. Ngemva kokugeza, i-alkaline phosphataselabeled murine anti-human IgM monoclonal antibody yengezwa esinyathelweni sesibili ukuze kwakheke inhlayiya kazibuthe-nucleoprotein_anti-2019-nCoV-IgM murine-anti-IgM-ALP-enzyme reaction reaction. Ngemuva komunye umjikelezo wokugeza, i-substrate ye-alkaline phosphatase yengezwa engxubeni yokusabela. Ukusabela kwe-chemiluminescence okuwumphumela bese kukalwa ngamayunithi okukhanya okuhlobene (RLU). Ukugxila kwe-anti-2019-nCoV -IgM antibody kusampula ilingana nenani lamayunithi okukhanya ahlobene (RLU) engxube. Ngakho-ke umphumela ungasetshenziswa ukuthola ubukhona be-IgM kusampula kanye nosizo lokunquma ukuthi isiguli sithelelekile yini igciwane lika-2019-nCoV.