【Izimiso Zenqubo】

I-2019-nCOV IgG Antibody Assay Reagent Kit (CMIA) iyizinyathelo ezimbili-ukuhlolwa kokuzivikela komzimba kusetshenziswa ubuchwepheshe bezinhlayiyana kazibuthe chemiluminescence. Ingakwazi ukucacisa ngokunembile ukuba khona kwamasosha omzimba e-anti-2019-nCoVIgG ku-serum yomuntu noma ku-plasma.

Ngesikhathi sokusabela, isinyathelo sokuqala ukubopha amaprotheni ngokuxuba isampula nezinhlayiya eziqinile ze-paramagnetic ezimbozwe nge-antigen ye-nuclear protein 2019-nCoV. Ngemva kokugeza, i-alkaline phosphataselabeled murine anti-human IgG monoclonal antibody yengezwa esinyathelweni sesibili ukuze kwakheke inhlayiya kazibuthe-nucleoprotein_anti-2019-nCoV -IgG_murine-anti-IgG- I-ALP-ingxube yokusabela kwe-enzyme. Ngemuva komunye umjikelezo wokugeza, i-substrate ye-alkaline phosphatase yengezwa engxubeni yokusabela. Ukusabela kwe-chemiluminescence okuwumphumela bese kukalwa ngamayunithi okukhanya okuhlobene (RLU). Ukugxila kwe-anti-2019-nCoV -IgG antibody kusampula ilingana nenani lamayunithi okukhanya ahlobene (RLU) wengxube. Ngakho-ke umphumela ungasetshenziswa ukuthola ubukhona be-IgG kusampula kanye nosizo lokunquma ukuthi isiguli singenwe yini igciwane lika-2019-nCoV.