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2019-nCOV IgG Antibody Assay Reagent Kit (CMIA)

Short Description:

The 2019-nCOV-IgG Assay Reagent Kit (CMIA) is a chemiluminescent microparticle immunoassay

(CMIA) for the qualitative determination of COVID-19/2019-nCOV IgG antibody in human serum as an aid in the diagnosis of 2019-nCOV infections.


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Principles of the Procedure

The 2019-nCOV IgG Antibody Assay Reagent Kit (CMIA) is a two-step immunoassay using magnetic particle chemiluminescence technology. It can precisely determine the presence of anti-2019-nCoVIgG antibodies in human serum or plasma.

During the reaction, the first step is protein binding by mixing the sample with strong paramagnetic particles coated with the 2019-nCoV nuclear protein antigen. After washing, the alkaline phosphataselabeled murine anti-human IgG monoclonal antibody is added in the second step to form a magnetic particle-nucleoprotein_anti-2019-nCoV -IgG_murine-anti-IgG- ALP-enzyme reaction mixture. After another wash cycle, the substrate of the alkaline phosphatase is added to the reaction mixture. The resulting chemiluminescence reaction is then measured in relative light units (RLU). The concentration of the anti-2019-nCoV -IgG antibody in the sample is proportional to the amount of relative light units (RLU) of the mixture. So the result can be used to detect the existence of the IgG in the sample and aid to determine if the patient has been infected by the 2019-nCoV virus.

Test Type

System

Incubation Time

Time to first result

Temperature

Sample

Volume

Immunometric

RL C2000

10 minutes

39 minutes

37 °C

50 μ


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